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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Leucine-Rich Glioma-Inactivated 1 (LGI1) Protein Stimulates Proliferation and IL-10 Production in Peripheral Blood Mononuclear Cells of Patients with LGI1 Antibody-Mediated Autoimmune Encephalitis In Vitro
doi: 10.3390/ijms25052581
Figure Lengend Snippet: Native and denatured LGI1 induce production and secretion of IL-10 in PBMCs from patients with LGI1 LE but not those from healthy controls. PBMCs of 4 patients with LGI1 LE (grey bars) and 10 healthy control volunteers (white bars) were incubated with native or denatured recombinant LGI1 or with cell culture medium and supernatant of non-transfected HEK 293 cells as vehicle controls. Cell culture supernatants were harvested after 4 days, and cytokine concentrations were determined with specific ELISA. IFN-γ, IL-5, IL-10, and IL-17 production are shown as means + SD; *** p < 0.001. Raw data are shown in .
Article Snippet: The concentrations of IFN-γ (#DIF50C),
Techniques: Incubation, Recombinant, Cell Culture, Transfection, Enzyme-linked Immunosorbent Assay